Validation Data Gallery

Filter: AllWBIPIHCIF-P

WB analysis using 12406-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12406-1-AP (Aconitase 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 12406-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12406-1-AP (Aconitase 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 12406-1-AP
IP result of anti-Aconitase 1 (IP:12406-1-AP, 4ug; Detection:12406-1-AP 1:5000) with HepG2 cells lysate 1925 ug.
IP experiment of HepG2 using 12406-1-AP
IP result of anti-Aconitase 1 (IP:12406-1-AP, 4ug; Detection:12406-1-AP 1:5000) with HepG2 cells lysate 1925 ug.
IHC staining of human stomach using 12406-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12406-1-AP (Aconitase 1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse liver using 12406-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Aconitase 1 antibody (12406-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

Aconitase 1 Polyclonal antibody

Aconitase 1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 12406-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, RIP, ELISA

Synonyms

ACO1, ACO 1, EC:4.2.1.3, Citrate hydro-lyase, Aconitase1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse liver tissue, rat liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse liver tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 11 publications below
IHC	See 2 publications below
IP	See 1 publications below
RIP	See 2 publications below

Product Information

12406-1-AP targets Aconitase 1 in WB, IHC, IF-P, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3083 Product name: Recombinant human ACO1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 540-890 aa of BC018103 Sequence: GRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALATPSDKLFFWNSKSTYIKSPPFFENLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAVMARGTFANIRLLNRFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQVKLDTGKTFQAVMRFDTDVELTYFLNGGILNYMIRKMAK 相同性解析による交差性が予測される生物種
Full Name	aconitase 1, soluble
Calculated molecular weight	889 aa, 98 kDa
Observed molecular weight	98 kDa
GenBank accession number	BC018103
Gene Symbol	Aconitase 1
Gene ID (NCBI)	48
RRID	AB_10642942
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P21399
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Mammalian cells possess two distinct isoforms of aconitase encoded by different genes: Aconitase 1 [ACO1; cytosolic aconitase (c-aconitase)] encoded by ACO1 and Aconitase 2 [ACO2; mitochondrial aconitase (m-aconitase)] encoded by ACO2. In addition to the enzymatic roles in the cytoplasm, ACO1 also is known to bind to mRNAs of specific genes and maintain iron homeostasis.

Protocols

Product Specific Protocols
IF protocol for Aconitase 1 antibody 12406-1-AP	Download protocol
IHC protocol for Aconitase 1 antibody 12406-1-AP	Download protocol
IP protocol for Aconitase 1 antibody 12406-1-AP	Download protocol
WB protocol for Aconitase 1 antibody 12406-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	iScience Asparagine starvation suppresses histone demethylation through iron depletion Authors - Jie Jiang KD Validated View Article
mouse	WB	Biochim Biophys Acta Mol Basis Dis Iron deposition-induced ferroptosis in alveolar type II cells promotes the development of pulmonary fibrosis. Authors - Haipeng Cheng View Article
human,mouse	WB,IHC	Cell Biosci Mutant huntingtin induces iron overload via up-regulating IRP1 in Huntington's disease. Authors - Li Niu View Article
human	WB	Drug Des Devel Ther Curcumin induces apoptosis and protective autophagy in castration-resistant prostate cancer cells through iron chelation. Authors - Chunguang Yang View Article
human	WB	Biomolecules Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite. Authors - Sara Ceccacci View Article
human	WB	JOJ Ophthalmol Mitophagy, Ferritinophagy and Ferroptosis in Retinal Pigment Epithelial Cells Under High Glucose Conditions: Implications for Diabetic Retinopathy and Age-Related Retinal Diseases. Authors - Lalit Pukhrambam Singh View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12406-1-AP

WB analysis using 12406-1-AP

IP experiment of HepG2 using 12406-1-AP

IP experiment of HepG2 using 12406-1-AP

IHC staining of human stomach using 12406-1-AP

IF Staining of mouse liver using 12406-1-AP

Aconitase 1 Polyclonal antibody

Aconitase 1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Asparagine starvation suppresses histone demethylation through iron depletion

Iron deposition-induced ferroptosis in alveolar type II cells promotes the development of pulmonary fibrosis.

Mutant huntingtin induces iron overload via up-regulating IRP1 in Huntington's disease.

Curcumin induces apoptosis and protective autophagy in castration-resistant prostate cancer cells through iron chelation.

Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite.

Mitophagy, Ferritinophagy and Ferroptosis in Retinal Pigment Epithelial Cells Under High Glucose Conditions: Implications for Diabetic Retinopathy and Age-Related Retinal Diseases.