• Featured Product
  • KD/KO Validated

ACADM Polyclonal antibody

ACADM Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55210-1-AP

Synonyms

ACAD1, ACADM, MCAD, MCADH



Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, HepG2 cells, rat heart tissue, MDA-MB-453s cells, mouse liver tissue, rat liver tissue, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman liver tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55210-1-AP targets ACADM in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
Calculated molecular weight 47 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberNM_000016
Gene symbol ACADM
Gene ID (NCBI) 34
RRIDAB_10837361
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.

Protocols

Product Specific Protocols
WB protocol for ACADM antibody 55210-1-APDownload protocol
IHC protocol for ACADM antibody 55210-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Res

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

Authors - Yunzi Mao
mouseIHC

Nat Cell Biol

BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma.

Authors - Jin-Tao Li
mouseWB

Circ Res

NAD+ Repletion Reverses Heart Failure With Preserved Ejection Fraction.

Authors - Dan Tong
mouseWB

Redox Biol

Increased fatty acid metabolism attenuates cardiac resistance to β-adrenoceptor activation via mitochondrial reactive oxygen species: A potential mechanism of hypoglycemia-induced myocardial injury in diabetes.

Authors - Lishan Huang
mouseWB

Cell Biosci

Hif-2α regulates lipid metabolism in alcoholic fatty liver disease through mitophagy

Authors - Mei-Fei Wu
humanWB

EMBO Rep

Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.

Authors - Yang Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yasuyo (Verified Customer) (07-22-2021)

Works good consistently.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:150
  • Cell Tissue Type: liver
ACADM Antibody Immunohistochemistry validation (1:150 dilution) in liver (Cat no:55210-1-AP)
FH

chao (Verified Customer) (01-30-2020)

Single strong, clear band for heart lysates

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart