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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    WB analysis of human plasma using 14181-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    WB analysis of BxPC-3 using 14181-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    WB analysis of MCF-7 using 14181-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    WB analysis of mouse kidney using 14181-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    IHC staining of human hepatocirrhosis using 14181-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14181-1-AP (Alpha 1B-Glycoprotein antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    IHC staining of human hepatocirrhosis using 14181-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14181-1-AP (Alpha 1B-Glycoprotein antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    IF Staining of HepG2 using 14181-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:25 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

Alpha 1B-Glycoprotein Polyclonal antibody

Alpha 1B-Glycoprotein Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14181-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

A1BG, A1B, Alpha-1-B glycoprotein, Alpha 1B glycoprotein, alpha 1 B glycoprotein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman plasma tissue, BxPC-3 cells, MCF-7 cells, mouse kidney tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14181-1-AP targets Alpha 1B-Glycoprotein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5414

Product name: Recombinant human A1BG protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-373 aa of BC035719

Sequence: MAPVSWITPGLKTTAVCRGVLRGVTFLLRREGDHEFLEVPEAQEDVEATFPVHQPGNYSCSYRTDGEGALSEPSATVTIEELAAPPPPVLMHHGESSQVLHPGNKVTLTCVAPLSGVDFQLRRGEKELLVPRSSTSPDRIFFHLNAVALGDGGHYTCRYRLHDNQNGWSGDSAPVELILSDETLPAPEFSPEPESGRALRLRCLAPLEGARFALVREDRGGRRVHRFQSPAGTEALFELHNISVADSANYSCVYVDLKPPFGGSAPSERLELHVDGPPPRPQLRATWSGAVLAGRDAVLRCEGPIPDVTFELLREGETKAVKTVRTPGAAANLELIFVGPQHAGNYRCRYRSWVPHTFESELSDPVELLVAES

相同性解析による交差性が予測される生物種
Full Name alpha-1-B glycoprotein
Calculated molecular weight 54 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC035719
Gene Symbol A1BG
Gene ID (NCBI) 1
RRIDAB_10640441
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP04217
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).

Protocols

Product Specific Protocols
IF protocol for Alpha 1B-Glycoprotein antibody 14181-1-APDownload protocol
IHC protocol for Alpha 1B-Glycoprotein antibody 14181-1-APDownload protocol
WB protocol for Alpha 1B-Glycoprotein antibody 14181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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