6*his,His tag Polyclonal antibody
6*his,His tag Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
recombinant protein
Applications
WB, IP, IF, ELISA
Conjugate
Unconjugated
Cat no : 10001-0-AP
Synonyms
"6*his,His tag Antibodies" Comparison
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Tested Applications
| Positive WB detected in | Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 15 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
10001-0-AP targets 6*his,His tag in WB, IP, IF, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Protein |
| Full Name | 6*his,His tag |
| Calculated molecular weight | 1 kDa |
| Gene symbol | |
| Gene ID (NCBI) | |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts specifically with the 6*His tag fused to recombinant protein.
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis TRIM25 regulates oxaliplatin resistance in colorectal cancer by promoting EZH2 stability. | ||
Cell Death Dis KAT2A-mediated AR translocation into nucleus promotes abiraterone-resistance in castration-resistant prostate cancer. | ||
J Vet Diagn Invest Preparation, identification, and functional analysis of monoclonal antibodies against atypical porcine pestivirus NS3 protein. | ||
Arch Insect Biochem Physiol The stability and antiapoptotic activity of Bm30K-3 can be improved by lysine acetylation in the silkworm, Bombyx mori. | ||
Arch Insect Biochem Physiol The acetylation modification regulates the stability of Bm30K-15 protein and its mechanism in silkworm, Bombyx mori. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Jean-Baptiste (Verified Customer) (04-21-2022) | Tested in Western blot for analysing the production in bacteria and the purification of a recombinant his-tagged protein. Worked quite well, with few unspecific bands.
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