6*his,His tag Polyclonal antibody

6*his,His tag Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


recombinant protein





Cat no : 10001-0-AP


6 his, 6*his,His tag, his tag

"6*his,His tag Antibodies" Comparison

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  • 6*his,His tag Antibody WB recombinant protein  10001-0-AP

    WB analysis of recombinant protein using 10001-0-AP

    Western blot of 6*His-tagged fusion protein with anti-6*HIS tag (10001-0-AP) at various dilutions.

Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

10001-0-AP targets 6*his,His tag in WB, IP, IF, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant Protein
Full Name 6*his,His tag
Calculated molecular weight 1 kDa
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts specifically with the 6*His tag fused to recombinant protein.



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Jean-Baptiste (Verified Customer) (04-21-2022)

Tested in Western blot for analysing the production in bacteria and the purification of a recombinant his-tagged protein. Worked quite well, with few unspecific bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
6*his,His tag Antibody Western Blot validation (1:2000 dilution) in (Cat no:10001-0-AP)