6*his,His tag Polyclonal antibody

6*his,His tag Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 10001-0-AP

Synonyms

6 his, 6*his,His tag, his tag


"6*his,His tag Antibodies" Comparison

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Applications:
  • 6*his,His tag Antibody WB recombinant protein  10001-0-AP

    WB analysis of recombinant protein using 10001-0-AP

    Western blot of 6*His-tagged fusion protein with anti-6*HIS tag (10001-0-AP) at various dilutions.


Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

10001-0-AP targets 6*his,His tag in WB, IP, IF, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant Protein
Full Name 6*his,His tag
Calculated molecular weight 1 kDa
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts specifically with the 6*His tag fused to recombinant protein.

Publications

SpeciesApplicationTitle
WB

Cell Death Dis

TRIM25 regulates oxaliplatin resistance in colorectal cancer by promoting EZH2 stability.

Authors - Sha Zhou
WB

Cell Death Dis

KAT2A-mediated AR translocation into nucleus promotes abiraterone-resistance in castration-resistant prostate cancer.

Authors - Dingheng Lu
WB

Protein J

Crystal structure of Human ASB9-2 and substrate-recognition of CKB.

Authors - Fei Xiangwei X
WB

J Vet Diagn Invest

Preparation, identification, and functional analysis of monoclonal antibodies against atypical porcine pestivirus NS3 protein.

Authors - Miaomiao Yan

Arch Insect Biochem Physiol

The stability and antiapoptotic activity of Bm30K-3 can be improved by lysine acetylation in the silkworm, Bombyx mori.

Authors - Yafei Ma
WB

Arch Insect Biochem Physiol

The acetylation modification regulates the stability of Bm30K-15 protein and its mechanism in silkworm, Bombyx mori.

Authors - Jiao Lv

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jean-Baptiste (Verified Customer) (04-21-2022)

Tested in Western blot for analysing the production in bacteria and the purification of a recombinant his-tagged protein. Worked quite well, with few unspecific bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
6*his,His tag Antibody Western Blot validation (1:2000 dilution) in (Cat no:10001-0-AP)