HRP-conjugated 6*His, His-Tag Monoclonal antibody

6*His, His-Tag Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1


recombinant protein


WB, ELISA, Blocking assay


HRP Fluorescent Dye

Cat no : HRP-66005


6*His, 6*His, His Tag, 6*His, His-Tag, 6histag, 6XHis, HHHHHH, His tag, HISTIDINE TAGGED, HRP 6XHis, HRP HHHHHH, HRP His tag

Tested Applications

Positive WB detected inRecombinant protein protein

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

HRP-66005 targets 6*His, His-Tag in WB, ELISA, Blocking assay applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen 6xHis Peptide
Full Name 6*His, His-Tag
Calculated molecular weight 1 kDa
Gene symbol
Gene ID (NCBI)
Conjugate HRP Fluorescent Dye
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. This His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells. This antibody is conjugated with HRP.

Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.


Product Specific Protocols
WB protocol for HRP 6*His, His-Tag antibody HRP-66005Download protocol
Standard Protocols
Click here to view our Standard Protocols



PLoS One

PMID: 30677074

Authors - Elizabeth C Upton

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Cell Mol Life Sci

Characterization of PPIB interaction in the P3H1 ternary complex and implications for its pathological mutations.

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J Biol Chem

Characterization of two engineered dimeric Zika virus envelope proteins as immunogen for neutralizing antibody selection and vaccine design.

Authors - Chunpeng Yang

Anal Chem

Receptor-Mediated Field Effect Transistor Biosensor for Real-Time Monitoring of Glutamate Release from Primary Hippocampal Neurons.

Authors - Yu-Tao Li


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tom (Verified Customer) (07-12-2021)

Blocked membrane overnight in 5% BSA in TBS. Tried at both 1:1000 and 1:10,000 dilutions in 5% BSA in TBST for 2.5h at room temperature and got great, specific bands for both dilutions.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000 & 1:10,000
  • Cell Tissue Type: IMAC Purified Bacterial Protein Lysate

Apoorv (Verified Customer) (12-15-2019)

Used to detect protein in Western blots

  • Applications: Western Blot,

Soo (Verified Customer) (10-24-2019)

we use this antibody to detect recombinant proteins which has his tag. Used it for western and ELISA and Flow , works well for us. It is cheaper than other brand's his tag antibody, another reason we like this product.

  • Applications: Western Blot, Flow Cytometry, Other,
  • Primary Antibody Dilution: 1:1000

Angel (Verified Customer) (09-09-2019)

It is the best antibody I've ever used for quick check of protein expression and size for heterologous protein production and purification.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:25000

Ryan (Verified Customer) (02-04-2019)

This His-tag-HRP conjugated antibody is the single best His-tag antibody I have ever used. I have used dilutions from 1:1000 to 1:25,000 with fantastic results. The background with this antibody is minimal and the sensitivity is superb. This image shows four different proteins with both 6xHis and 8xHis-tags. I have used the antibody in milk and 5% BSA. Incubation times have been as short as 1 hour and as long as 24 hours; the results are always the same. Being conjugated, it can cut the time of a typical Western blot down by a few hours. My standard procedure with this antibody is 1:8,000 in 5% BSA for 1 hour with three 10 minute washes and a 5 second exposure using Cell Signaling SignalFire ECL.

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 1:8000
  • Cell Tissue Type: Expi293 lysate
HRP 6*His, His-Tag Antibody Western Blot,Immunoprecipitation, validation (1:8000 dilution) in Expi293 lysate (Cat no:HRP-66005)

Jennifer (Verified Customer) (12-08-2018)

Excellent product. We achieved to see the production of our protein of interest in an accurate way. Some previous secondary antibodies gave us a lot of background; this one works better.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/5000
  • Cell Tissue Type: lysate