HRP-conjugated 6*His, His-Tag Monoclonal antibody
6*His, His-Tag Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG1
Reactivity
recombinant protein
Applications
WB, ELISA, Blocking assay
Conjugate
HRP Fluorescent Dye
Cat no : HRP-66005
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Recombinant protein protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 33 publications below |
| ELISA | See 1 publications below |
Product Information
HRP-66005 targets 6*His, His-Tag in WB, ELISA, Blocking assay applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | 6xHis Peptide |
| Full Name | 6*His, His-Tag |
| Calculated molecular weight | 1 kDa |
| Gene symbol | |
| Gene ID (NCBI) | |
| Conjugate | HRP Fluorescent Dye |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. This His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells. This antibody is conjugated with HRP.
Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP 6*His, His-Tag antibody HRP-66005 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Differ STING signaling remodels the tumor microenvironment by antagonizing myeloid-derived suppressor cell expansion. | ||
J Biol Chem The retroviral accessory proteins S2, Nef, and glycoMA use similar mechanisms for antagonizing the host restriction factor SERINC5. | ||
Cell Mol Life Sci Characterization of PPIB interaction in the P3H1 ternary complex and implications for its pathological mutations. | ||
J Biol Chem Characterization of two engineered dimeric Zika virus envelope proteins as immunogen for neutralizing antibody selection and vaccine design. | ||
Anal Chem Receptor-Mediated Field Effect Transistor Biosensor for Real-Time Monitoring of Glutamate Release from Primary Hippocampal Neurons. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tom (Verified Customer) (07-12-2021) | Blocked membrane overnight in 5% BSA in TBS. Tried at both 1:1000 and 1:10,000 dilutions in 5% BSA in TBST for 2.5h at room temperature and got great, specific bands for both dilutions.
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FH Apoorv (Verified Customer) (12-15-2019) | Used to detect protein in Western blots
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FH Soo (Verified Customer) (10-24-2019) | we use this antibody to detect recombinant proteins which has his tag. Used it for western and ELISA and Flow , works well for us. It is cheaper than other brand's his tag antibody, another reason we like this product.
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FH Angel (Verified Customer) (09-09-2019) | It is the best antibody I've ever used for quick check of protein expression and size for heterologous protein production and purification.
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FH Ryan (Verified Customer) (02-04-2019) | This His-tag-HRP conjugated antibody is the single best His-tag antibody I have ever used. I have used dilutions from 1:1000 to 1:25,000 with fantastic results. The background with this antibody is minimal and the sensitivity is superb. This image shows four different proteins with both 6xHis and 8xHis-tags. I have used the antibody in milk and 5% BSA. Incubation times have been as short as 1 hour and as long as 24 hours; the results are always the same. Being conjugated, it can cut the time of a typical Western blot down by a few hours. My standard procedure with this antibody is 1:8,000 in 5% BSA for 1 hour with three 10 minute washes and a 5 second exposure using Cell Signaling SignalFire ECL.
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FH Jennifer (Verified Customer) (12-08-2018) | Excellent product. We achieved to see the production of our protein of interest in an accurate way. Some previous secondary antibodies gave us a lot of background; this one works better.
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