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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Cytokeratin 5 Monoclonal antibody

Cytokeratin 5 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A1C5

Cat no : CL488-66727

Synonyms

58 kDa cytokeratin, Cell and organelle markers, CK 5, CK5, Cytokeratin 5, Cytoskeleton Marker, K5, keratin 5, KRT5, KRT5A, Type II keratin Kb5

Validation Data Gallery

Applications:
  • IF Staining of human oesophagus cancer using CL488-66727

    IF Staining of human oesophagus cancer using CL488-66727

    Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using CoraLite® Plus 488 Cytokeratin 5 antibody (CL488-66727, Clone: 1A1C5 ) at dilution of 1:200.

  • IF Staining of human oesophagus cancer using CL488-66727

    IF Staining of human oesophagus cancer using CL488-66727

    Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using CoraLite® Plus 488 Cytokeratin 5 antibody (CL488-66727, Clone: 1A1C5 ) at dilution of 1:200.

Tested Applications

Positive IF detected inhuman oesophagus cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 66727-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66727 targets Cytokeratin 5 in IF applications and shows reactivity with Human, mouse, pig samples.

Tested Reactivity Human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 5 fusion protein Ag24184 相同性解析による交差性が予測される生物種
Full Name keratin 5
Calculated molecular weight 590 aa, 62 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC024292
Gene symbol KRT5
Gene ID (NCBI) 3852
RRIDAB_2919361
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD keratin 14 in stratified squamous epithelia. Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD tyK14 in stratified squamous epithelia.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 5 antibody CL488-66727Download protocol
Standard Protocols
Click here to view our Standard Protocols