Cytokeratin 5 Monoclonal antibody

Cytokeratin 5 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1A1C5

Cat no : 66727-1-Ig

Synonyms

58 kDa cytokeratin, Cell and organelle markers, CK 5, CK5, Cytokeratin 5, Cytoskeleton Marker, K5, keratin 5, KRT5, KRT5A, Type II keratin Kb5



Tested Applications

Positive WB detected inHaCaT cells, SKOV-3 cells, mouse skin tissue, MCF-7 cells, A549 cells, A-253 cells, A431 cells, human saliva, pig skin tissue
Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue, human breast hyperplasia tissue, human lung cancer tissue, human oesophagus cancer tissue, human cervical cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells, human oesophagus cancer tissue, human prostate cancer tissue
Positive FC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

66727-1-Ig targets Cytokeratin 5 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, pig samples.

Tested Reactivity Human, mouse, pig
Cited Reactivityhuman, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 5 fusion protein Ag24184 相同性解析による交差性が予測される生物種
Full Name keratin 5
Calculated molecular weight 590 aa, 62 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC024292
Gene symbol KRT5
Gene ID (NCBI) 3852
RRIDAB_2882077
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD keratin 14 in stratified squamous epithelia. Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD tyK14 in stratified squamous epithelia.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 5 antibody 66727-1-IgDownload protocol
IHC protocol for Cytokeratin 5 antibody 66727-1-IgDownload protocol
IF protocol for Cytokeratin 5 antibody 66727-1-IgDownload protocol
FC protocol for Cytokeratin 5 antibody 66727-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Cell Mol Biol Lett

The role of NPY2R/NFATc1/DYRK1A regulatory axis in sebaceous glands for sebum synthesis

Authors - Tao Yang
mouseIHC

Front Genet

The Unique Genetic and Histological Characteristics of DMBA-Induced Mammary Tumors in an Organoid-Based Carcinogenesis Model.

Authors - Mie Naruse
humanWB

PLoS One

Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.

Authors - Kui Xiao
humanWB

Cell Death Dis

SOX1 promotes differentiation of nasopharyngeal carcinoma cells by activating retinoid metabolic pathway.

Authors - Xin-Xing Lei
humanWB

Cell Death Discov

TRIM29 hypermethylation drives esophageal cancer progression via suppression of ZNF750

Authors - Qiyi Yi
mouseIHC

Immunobiology

High expression of NF-κB inducing kinase in the bulge region of hair follicle induces tumor

Authors - Weisong Duan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Emma (Verified Customer) (11-29-2021)

Used by IF on FFPE prostate tissue. Tris-EFTA antigen retrieval.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Prostate
Cytokeratin 5 Antibody Immunofluorescence validation (1:100 dilution) in Prostate (Cat no:66727-1-Ig)