pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 60331-1-Ig (NF-H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 60331-1-Ig (NF-H antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NF-H/NF200 antibody (60331-1-Ig, Clone: 1A3C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.
1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NF-H/NF200 (60331-1-Ig, Clone:1A3C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of SH-SY5Y cells using 60331-1-Ig (NF200 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.
