1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD127 (IL-7Ra) (65093-1-Ig, Clone: A7R34) or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-rat IgG2a Antibody at dilution 1:100. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.