pan-keratin Polyclonal antibody
pan-keratin Polyclonal Antibody for WB, IF, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IF, FC, IHC, ELISA
Conjugate
Unconjugated
Cat no : 26411-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, MCF-7 cells, HeLa cells |
Positive IHC detected in | human oesophagus tissue, human liver tissue, human lung cancer tissue, human renal cell carcinoma tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human colon cancer tissue, HeLa cells, A431 cells |
Positive FC detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:50000-1:200000 |
Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 3 publications below |
IHC | See 12 publications below |
IF | See 13 publications below |
Product Information
26411-1-AP targets pan-keratin in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, rat, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | pan-keratin fusion protein Ag24184 相同性解析による交差性が予測される生物種 |
Full Name | keratin 5 |
Calculated molecular weight | 590 aa, 62 kDa |
Observed molecular weight | 46-58 kDa |
GenBank accession number | BC024292 |
Gene symbol | KRT5 |
Gene ID (NCBI) | 3852 |
RRID | AB_2880505 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers. Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases. This antibody is a pan-keratin antibody.
Protocols
Product Specific Protocols | |
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WB protocol for pan-keratin antibody 26411-1-AP | Download protocol |
IHC protocol for pan-keratin antibody 26411-1-AP | Download protocol |
IF protocol for pan-keratin antibody 26411-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biomaterials Heterotypic 3D pancreatic cancer model with tunable proportion of fibrotic elements. | ||
Biomaterials MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system. | ||
Oncogene The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer | ||
Oncogene USP13 promotes development and metastasis of high-grade serous ovarian carcinoma in a novel mouse model. | ||
Front Cell Dev Biol Preclinical Application of Conditional Reprogramming Culture System for Laryngeal and Hypopharyngeal Carcinoma | ||
J Cancer Res Clin Oncol B7-H3 and CD47 co-expression in gastric cancer is a predictor of poor prognosis and potential targets for future dual-targeting immunotherapy |