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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated YTHDF2 Polyclonal antibody

YTHDF2 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-24744

Synonyms

CLL associated antigen KW 14, HGRG8, NY REN 2, YTH domain family protein 2, YTH domain family, member 2, YTHDF2

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL488-24744

    FC experiment of HeLa using CL488-24744

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human YTHDF2 (CL488-24744) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy YTHDF2 Ready-To-Use IHC Kit. YTHDF2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 24744-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-24744 is YTHDF2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YTHDF2 fusion protein Ag5922 相同性解析による交差性が予測される生物種
Full Name YTH domain family, member 2
Calculated molecular weight 62 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC002559
Gene symbol YTHDF2
Gene ID (NCBI) 51441
RRIDAB_2919200
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

YTHDF2 also named as CLL associated antigen KW 14 or HGRG8 is a 579 amino acid protein, which contains one YTH domain and exists as two alternatively spliced isoforms. YTHDF2 is expressed in in pancreas, testis and placenta. YTHDF2 may play a role in human longevity.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 YTHDF2 antibody CL488-24744Download protocol
Standard Protocols
Click here to view our Standard Protocols