CoraLite® Plus 488-conjugated Synaptopodin Polyclonal antibody
Synaptopodin Polyclonal Antibody for IF, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-21064
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | rat brain tissue |
Positive FC detected in | U-87 MG cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-21064 targets Synaptopodin in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synaptopodin fusion protein Ag15089 相同性解析による交差性が予測される生物種 |
Full Name | synaptopodin |
Calculated molecular weight | 929 aa, 99 kDa |
Observed molecular weight | 99-115 kDa |
GenBank accession number | BC146665 |
Gene symbol | SYNPO |
Gene ID (NCBI) | 11346 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 Synaptopodin antibody CL488-21064 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |