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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SURF1 Recombinant antibody

SURF1 Recombinant Antibody for IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230210D5

Cat no : 83052-7-RR

Synonyms

SURF 1, SURF1, surfeit 1, Surfeit locus protein 1

Validation Data Gallery

Applications:
  • IHC staining of human liver cancer using 83052-7-RR

    IHC staining of human liver cancer using 83052-7-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 83052-7-RR (SURF1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 83052-7-RR

    IHC staining of human liver cancer using 83052-7-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 83052-7-RR (SURF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 83052-7-RR

    IHC staining of mouse heart using 83052-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83052-7-RR (SURF1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 83052-7-RR

    IHC staining of mouse heart using 83052-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83052-7-RR (SURF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 83052-7-RR

    IF Staining of HeLa using 83052-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SURF1 antibody (83052-7-RR, Clone: 230210D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • IF Staining of HeLa using 83052-7-RR

    IF Staining of HeLa using 83052-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SURF1 antibody (83052-7-RR, Clone: 230210D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 83052-7-RR

    FC experiment of HepG2 using 83052-7-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human SURF1 (83052-7-RR, Clone:230210D5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IHC detected inmouse heart tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

83052-7-RR targets SURF1 in IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SURF1 fusion protein Ag3959 相同性解析による交差性が予測される生物種
Full Name surfeit 1
Calculated molecular weight 33 kDa
Observed molecular weight30-31 kDa
GenBank accession numberBC028314
Gene symbol SURF1
Gene ID (NCBI) 6834
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for SURF1 antibody 83052-7-RRDownload protocol
IF protocol for SURF1 antibody 83052-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols