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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SIAE Monoclonal antibody

SIAE Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse , rat , pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G5F7

Cat no : CL488-66522

Synonyms

CSE C, H Lse, LSE, SIAE, Sialate O acetylesterase, sialic acid acetylesterase, YSG2

Validation Data Gallery

Applications:
  • FC experiment of PC-3 using CL488-66522

    FC experiment of PC-3 using CL488-66522

    1X10^6 PC-3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SIAE (CL488-66522, Clone:1G5F7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inPC-3 cells
For other applications, we recommend the unconjugated version of this antibody, 66522-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-66522 targets SIAE in FC (Intra) applications and shows reactivity with Human, mouse , rat , pig samples.

Tested Reactivity Human, mouse , rat , pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIAE fusion protein Ag14624 相同性解析による交差性が予測される生物種
Full Name sialic acid acetylesterase
Calculated molecular weight 58 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC068450
Gene symbol SIAE
Gene ID (NCBI) 54414
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 SIAE antibody CL488-66522Download protocol
Standard Protocols
Click here to view our Standard Protocols