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  • Featured Product
  • KD/KO Validated

RRM2B,p53R2 Polyclonal antibody

RRM2B,p53R2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18005-1-AP

Synonyms

p53R2, RRM2B, RRM2B,p53R2

Validation Data Gallery

Applications:
  • WB analysis using 18005-1-AP

    WB analysis using 18005-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 18005-1-AP

    WB analysis of PC-3 using 18005-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 18005-1-AP

    WB analysis of PC-3 using 18005-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 18005-1-AP

    WB analysis of HeLa using 18005-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of MDA-MB-453s using 18005-1-AP

    WB analysis of MDA-MB-453s using 18005-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA431 cells, PC-3 cells, MDA-MB-453s cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

18005-1-AP targets RRM2B,p53R2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRM2B,p53R2 fusion protein Ag12499 相同性解析による交差性が予測される生物種
Full Name ribonucleotide reductase M2 B (TP53 inducible)
Calculated molecular weight 351 aa, 41 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC108261
Gene symbol RRM2B
Gene ID (NCBI) 50484
RRIDAB_2878480
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RRM2B,p53R2 antibody 18005-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Ther

DGCR8/ZFAT-AS1 Promotes CDX2 Transcription in a PRC2 Complex-Dependent Manner to Facilitate the Malignant Biological Behavior of Glioma Cells.

Authors - Fangfang Zhang
  • KD Validated
View Article