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Progranulin/PGRN Polyclonal antibody
Progranulin/PGRN Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 18410-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, A431 cells, HeLa cells, HEK-293 cells, PC-3 cells, human saliva, mouse heart tissue, mouse lung tissue, rat brain tissue, U-937 cells, THP-1 cells, RAW 264.7 cells |
Positive IHC detected in | human pancreas cancer tissue, human kidney tissue, human lung cancer tissue, human ovary tumor tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 7 publications below |
IHC | See 7 publications below |
IF | See 2 publications below |
Product Information
18410-1-AP targets Progranulin/PGRN in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, rat, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Progranulin/PGRN fusion protein Ag13178 相同性解析による交差性が予測される生物種 |
Full Name | granulin |
Calculated molecular weight | 64 kDa |
Observed molecular weight | 60-70 kDa |
GenBank accession number | BC000324 |
Gene symbol | Granulin |
Gene ID (NCBI) | 2896 |
RRID | AB_10598161 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system.
Protocols
Product Specific Protocols | |
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WB protocol for Progranulin/PGRN antibody 18410-1-AP | Download protocol |
IHC protocol for Progranulin/PGRN antibody 18410-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Transl Psychiatry Progranulin improves neural development via the PI3K/Akt/GSK-3β pathway in the cerebellum of a VPA-induced rat model of ASD. | ||
J Proteome Res Mining the gastric cancer secretome: identification of GRN as a potential diagnostic marker for early gastric cancer. | ||
J Mol Med (Berl) Progranulin alleviates podocyte injury via regulating CAMKK/AMPK-mediated autophagy under diabetic conditions. | ||
Front Oncol GRN is a prognostic biomarker and correlated with immune infiltration in glioma: A study based on TCGA data | ||
Neuropharmacology Abnormal spatiotemporal expression pattern of progranulin and neurodevelopment impairment in VPA-induced ASD rat model. | ||
Int J Mol Sci Involvement of Progranulin and Granulin Expression in Inflammatory Responses after Cerebral Ischemia. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sharan (Verified Customer) (06-24-2020) | The antibody detects the GRN protein but there is also a lot of cross-reactivity with non-specific antigens in HeLa lysate
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