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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

P85a; PIK3R1 Polyclonal antibody

P85a; PIK3R1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30092-1-AP

Synonyms

GRB1, p85, p85 ALPHA, PI3 kinase subunit p85 alpha, PI3K regulatory subunit alpha, PIK3R1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using P85a; PIK3R1 Polyclonal antibody (30092-1-AP)

    WB analysis using 30092-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30092-1-AP (P85a; PIK3R1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using P85a; PIK3R1 Polyclonal antibody (30092-1-AP)

    WB analysis using 30092-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30092-1-AP (P85a; PIK3R1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HepG2 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30092-1-AP targets P85a; PIK3R1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
Calculated molecular weight 85 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC030815
Gene symbol PI3 Kinase p85 Alpha
Gene ID (NCBI) 5295
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for P85a; PIK3R1 antibody 30092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols