Mouse IL12 p70 ELISA Kit0 Publications

カタログ番号: KE10014

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Mouse IL12 p70 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
2.2 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Mouse

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 87% 78%-105%
Mouse serum 101% 84%-115%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 821.4 55.5 6.8
2 20 191.8 6.4 3.4
3 20 19.7 1.1 5.4

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 776.8 52.9 6.8
2 24 191.5 12.0 6.2
3 24 18.7 1.1 6.0

製品概要
K10014 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL12 p70 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL12 p70. The assay recognizes mouse IL12 p70. A monoclonal antibody specific for mouse IL12 p70 has been pre-coated onto the microwells. The mouse IL12 p70 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for mouse IL12 p70 is added to detect the captured mouse IL12 p70 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Il 12a, IL 12p35, IL-12, Il12a, interleukin 12a, Interleukin-12, Ll12a, p35
背景説明

Interleukin-12 (IL12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL12 (referred to as 'p70') is a 70 kDa heterodimer composed of a 35 kDa subunit (IL12a p35) and a 40 kDa subunit (IL12b p40). IL12 is involved in the differentiation of native T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. IL12 plays an important role in the activities of natural killer cells and T lymphocytes. IL12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. IL12 has been shown to play a critical role in the pathogenesis of a variety of immune-related diseases.


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