Mouse GM-CSF ELISA Kit0 Publications

カタログ番号: KE10015

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Mouse GM-CSF ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
1.2 pg/mL

測定範囲:
4.7 - 300 pg/mL

測定可能動物種:
Mouse

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 98% 85%-119%
Mouse serum 85% 80%-97%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 32.7 1.8 5.5
2 20 80.8 2.9 3.6
3 20 304.4 19.9 6.6

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 30.2 1.6 5.4
2 24 75.3 5.5 7.3
3 24 306.0 17.9 5.9

製品概要
KE10015 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse GM-CSF ELISA kit is to be used to detect and quantify protein levels of endogenous mouse GM-CSF. The assay recognizes mouse GM-CSF. A monoclonal antibody specific for mouse GM-CSF has been pre-coated onto the microwells. The mouse GM-CSF protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for mouse GM-CSF is added to detect the captured mouse GM-CSF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Csf2, Csfgm, Gm CSf, GM-CSF, MGI IGM
背景説明

GM-CSF, also known as Csf2, is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. GM-CSF was first characterized as a hematopoietic growth factor that stimulates the proliferation of myeloid cells from bone-marrow progenitors. GM-CSF is now recognized as an important activating and differentiation factor for immune cells, and is essential for a wide range of biological processes in both innate and adaptive immunity. GM-CSF has been shown to protect against pulmonary infection and intestinal inflammation, and it is necessary for normal pulmonary and colon homeostasis.


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