MCP-1 Polyclonal antibody

MCP-1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26161-1-AP

Synonyms

AI323594, Ccl2, chemokine (C C motif) ligand 2, HC11, JE, MCAF, MCP 1, Mcp1, Scya2, Sigje, SMC CF


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Tested Applications

Positive WB detected inLPS and Brefeldin A treated RAW 264.7 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26161-1-AP targets MCP-1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCP-1 fusion protein Ag24085 相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) ligand 2
Observed molecular weight16-20 kDa
GenBank accession numberNM-011333
Gene symbol MCP-1
Gene ID (NCBI) 20296
RRIDAB_2918100
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.

Protocols

Product Specific Protocols
WB protocol for MCP-1 antibody 26161-1-APDownload protocol
IF protocol for MCP-1 antibody 26161-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC,IF

Acta Pharmacol Sin

Macrophages promote the transition from myocardial ischemia reperfusion injury to cardiac fibrosis in mice through GMCSF/CCL2/CCR2 and phenotype switching

Authors - Shi-Chun Shen
ratWB

Int J Mol Sci

Enhanced Activation of the S1PR2-IL-1β-Src-BDNF-TrkB Pathway Mediates Neuroinflammation in the Hippocampus and Cognitive Impairment in Hyperammonemic Rats

Authors - María Sancho-Alonso
mouseIF

Front Cell Neurosci

Effects of Ischemia on the Migratory Capacity of Microglia Along Collagen Microcontact Prints on Organotypic Mouse Cortex Brain Slices.

Authors - Katharina Steiner
mouseIHC

Pharmaceutics

Targeted Therapy of Acute Liver Injury via Cryptotanshinone-Loaded Biomimetic Nanoparticles Derived from Mesenchymal Stromal Cells Driven by Homing

Authors - Xin Zhang
mouseIF

J Biol Chem

rhFGF-21 accelerates corneal epithelial wound healing through the attenuation of oxidative stress and inflammatory mediators in diabetic mice

Authors - Le Li
mouseIF

Proc Natl Acad Sci U S A

Bacterial indole-3-lactic acid affects epithelium-macrophage crosstalk to regulate intestinal homeostasis

Authors - Kaiyuan Yu