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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated IFT20 Polyclonal antibody

IFT20 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Canine

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13615

Synonyms

hIFT20, IFT20

Validation Data Gallery

Applications:
  • IF Staining of hTERT-RPE1 using CL488-13615

    IF Staining of hTERT-RPE1 using CL488-13615

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite® Plus 488 IFT20 antibody (CL488-13615) at dilution of 1:200.

Tested Applications

Positive IF detected inhTERT-RPE1 cells
For other applications, we recommend the unconjugated version of this antibody, 13615-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-13615 targets IFT20 in IF applications and shows reactivity with human, mouse, Canine samples.

Tested Reactivity human, mouse, Canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT20 fusion protein Ag4521 相同性解析による交差性が予測される生物種
Full Name intraflagellar transport 20 homolog (Chlamydomonas)
Calculated molecular weight 15 kDa
Observed molecular weight 15-18 kDa
GenBank accession numberBC038094
Gene symbol IFT20
Gene ID (NCBI) 90410
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium. IFT particles are multi-subunit complexes that are made up of complex A and complex B. IFT20 is a component of IFT complex B and involved in ciliary process assembly. It is associated with the Golgi complex and plays a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 IFT20 antibody CL488-13615Download protocol
Standard Protocols
Click here to view our Standard Protocols