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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Huntingtin Monoclonal antibody

Huntingtin Monoclonal Antibody for FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2F6C9

Cat no : 66553-1-Ig

Synonyms

HD, HD protein, HTT, huntingtin, Huntington disease protein, IT15

Validation Data Gallery

Applications:
  • IHC staining of human cerebellum using 66553-1-Ig

    IHC staining of human cerebellum using 66553-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66553-1-Ig (Huntingtin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HTT antibody included
  • IHC staining of human cerebellum using 66553-1-Ig

    IHC staining of human cerebellum using 66553-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66553-1-Ig (Huntingtin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HTT antibody included
  • IHC staining of human brain using 66553-1-Ig

    IHC staining of human brain using 66553-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66553-1-Ig (Huntingtin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HTT antibody included
  • IHC staining of human brain using 66553-1-Ig

    IHC staining of human brain using 66553-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66553-1-Ig (Huntingtin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HTT antibody included
  • FC experiment of SH-SY5Y using 66553-1-Ig

    FC experiment of SH-SY5Y using 66553-1-Ig

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human Huntingtin (66553-1-Ig, Clone:2F6C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IHC detected inhuman cerebellum tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy HTT Ready-To-Use IHC Kit. HTT primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

66553-1-Ig targets Huntingtin in IHC, FC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Huntingtin fusion protein Ag25922 相同性解析による交差性が予測される生物種
Full Name huntingtin
Calculated molecular weight 348 kDa
GenBank accession numberNM_002111
Gene symbol HTT
Gene ID (NCBI) 3064
RRIDAB_2881915
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for Huntingtin antibody 66553-1-IgDownload protocol
FC protocol for Huntingtin antibody 66553-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols