Human PLA2G1B ELISA Kit0 Publications

カタログ番号: KE00098

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human PLA2G1B ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Cell culture supernatant, Cell lysate

アッセイ方法:
Sandwich

感度:
0.08 ng/mL

測定範囲:
0.094 - 96 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 105% 77%-126%
Cell lysate 100% 72%-115%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 0.69 0.05 6.7
2 20 9.55 0.87 9.1
3 20 92.38 9.37 10.1

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 0.64 0.04 6.9
2 24 9.45 0.59 6.3
3 24 78.98 6.95 8.8

製品概要
KE00098 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Human PLA2G1B ELISA kit is to be used to detect and quantify protein levels of endogenous human PLA2G1B. The assay recognizes human PLA2G1B. A monoclonal antibody specific for human PLA2G1B has been pre-coated onto the microwells. The human PLA2G1B protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for human PLA2G1B is added to detect the captured human PLA2G1B protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Group IB phospholipase A2, Phospholipase A2, PLA2, PLA2A, PLA2G1B, PPLA2
背景説明

Phospholipase A2 group IB, also know as PLA2G1B, is a secreted member of the phospholipase A2 (PLA2) class of enzymes, is produced by the pancreatic acinar cells. PLA2G1B is a calcium-dependent enzyme which can catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. PLA2G1B is involved in several physiological processes including cell contraction, cell proliferation and pathological response.


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