Human MMP-2 ELISA Kit
Cat no : KE00077
Synonyms
72 kDa gelatinase, 72 kDa type IV collagenase, CLG4, CLG4A, Gelatinase A, Matrix metalloproteinase 2, MMP 2, MMP II, MMP2, MMP-2, MONA, TBE 1
Validation Data Gallery
Product Information
KE00077 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MMP-2 ELISA kit is to be used to detect and quantify protein levels of endogenous MMP-2. The assay recognizes human MMP-2. An antibody specific for MMP-2 has been pre-coated onto the microwells. The MMP-2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for MMP-2 is added to detect the captured MMP-2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MMP-2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.625-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4313 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 116% | 104%-129% |
| Cell culture supernatants | 101% | 87%-115% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 15.24 | 0.68 | 4.5 |
| 2 | 20 | 4.99 | 0.27 | 5.4 |
| 3 | 20 | 0.65 | 0.07 | 10.0 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 16.01 | 1.20 | 7.5 |
| 2 | 24 | 4.71 | 0.26 | 5.4 |
| 3 | 24 | 0.83 | 0.07 | 8.9 |
Background Information
MMP-2 (also designated type IV collagenase) cleaves collagen types IV, V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP-2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 72 kDa gelatinase, 72 kDa type IV collagenase, CLG4, CLG4A, Gelatinase A, Matrix metalloproteinase 2, MMP 2, MMP II, MMP2, MMP-2, MONA, TBE 1 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Gynecol Oncol NCK1 promotes the angiogenesis of cervical squamous carcinoma via Rac1/PAK1/MMP2 signal pathway. | ||
Lab Invest Exosomes derived from hypoxic glioma deliver miR-1246 and miR-10b-5p to normoxic glioma cells to promote migration and invasion. | ||
J Clin Med Zinc Administration and Improved Serum Markers of Hepatic Fibrosis in Patients with Autoimmune Hepatitis. | ||
Anticancer Res Clinicopathological and Prognostic Analysis of PD-L1 and PD-L2 Expression in Surgically Resected Primary Tongue Squamous Cell Carcinoma. | ||
Front Neurol Association of plasma MMP-2 levels and prognosis of patients with intracerebral hemorrhage: a prospective cohort study |