Human ICAM-1 ELISA Kit0 Publications

カタログ番号: KE00061

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human ICAM-1 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
11.1 pg/mL

測定範囲:
46.88-3000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 94% 86%-120%
Human plasma 102% 75%-126%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 166.8 6.6 4.0
2 20 656.7 18.9 2.9
3 20 2,420.7 90.7 3.7

InterAssay:
Samplenmean (pg/mL)SDCV%
1 20 172.2 11.9 6.9
2 20 666.0 34.8 5.2
3 20 2,522.8 154.4 6.1

製品概要
KE00061 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ICAM-1 ELISA kit is to be used to detect and quantify protein levels of endogenous ICAM-1. The assay recognizes human ICAM-1. A polyclonal antibody specific for ICAM-1 has been pre-coated onto the microwells. The ICAM-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for ICAM-1 is added to detect the captured ICAM-1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
BB2, CD54, ICAM 1, ICAM1, ICAM-1, P3.58
背景説明

Intercellular adhesion molecule 1 (ICAM-1, also known as CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin. It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum. Soluble form of ICAM-1 (sICAM-1) has been measured in various body fluids, with increased levels being found in serum from patients with cardiovascular disease, cancer and autoimmune diseases.


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