Human GOLM1 ELISA Kit0 Publications

カタログ番号: KE00111

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human GOLM1 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
0.01 ng/mL

測定範囲:
0.156 - 10 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatants 102% 78%-118%
Human plasma 95% 77%-114%
Human serum 104% 86%-126%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 4.04 0.18 4.5
2 20 1.00 0.04 3.6
3 20 0.25 0.01 2.5

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 3.91 0.19 4.8
2 24 0.96 0.04 3.8
3 24 0.23 0.01 2.7

製品概要
KE00111 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GOLM1 ELISA kit is to be used to detect and quantify protein levels of endogenous GOLM1. The assay recognizes human GOLM1. A monoclonal antibody specific for GOLM1 has been pre-coated onto the microwells. The GOLM1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody specific for GOLM1 is added to detect the captured GOLM1 protein. For signal development, HRP-conjugated is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
bA379P1.3, C9orf155, FLJ22634, FLJ23608, golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2, GOLM1, GOLPH2, GP73, PSEC0257, UNQ686/PRO1326
背景説明

GOLM1 (also known as GP73 or GOLPH2) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLM1 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLM1 could be a promising serum marker for hepatocellular carcinoma.


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