CoraLite®594-conjugated HuR/ELAVL1 Monoclonal antibody
HuR/ELAVL1 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat
Applications
IF
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1A11H2
Cat no : CL594-66549
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | L02 cells |
Planning an IHC experiment? We recommend our IHCeasy HuR/ELAVL1 Ready-To-Use IHC Kit. HuR/ELAVL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66549-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL594-66549 targets HuR/ELAVL1 in IF applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | HuR/ELAVL1 fusion protein Ag2249 相同性解析による交差性が予測される生物種 |
| Full Name | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R) |
| Calculated molecular weight | 326 aa, 36 kDa |
| Observed molecular weight | 37 kDa |
| GenBank accession number | BC003376 |
| Gene symbol | ELAVL1 |
| Gene ID (NCBI) | 1994 |
| RRID | AB_2883602 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 HuR/ELAVL1 antibody CL594-66549 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
