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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated HuR/ELAVL1 Monoclonal antibody

HuR/ELAVL1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A11H2

Cat no : CL488-66549

Synonyms

ELAV like protein 1, ELAV1, ELAVL1, Hu antigen R, Hua, HuR, MelG

Validation Data Gallery

Applications:
  • IF Staining of L02 using CL488-66549

    IF Staining of L02 using CL488-66549

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using CoraLite® Plus 488-conjugated HuR/ELAVL1 antibody (CL488-66549, Clone: 1A11H2 ) at dilution of 1:100, CL594-Phalloidin (red).

  • IF Staining of HepG2 using CL488-66549

    IF Staining of HepG2 using CL488-66549

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HuR antibody (CL488-66549, Clone: 1A11H2 ) at dilution of 1:800.

Tested Applications

Positive IF detected inL02 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HuR/ELAVL1 Ready-To-Use IHC Kit. HuR/ELAVL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66549-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66549 targets HuR/ELAVL1 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HuR/ELAVL1 fusion protein Ag2249 相同性解析による交差性が予測される生物種
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
Calculated molecular weight 326 aa, 36 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC003376
Gene symbol ELAVL1
Gene ID (NCBI) 1994
RRIDAB_2883354
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HuR/ELAVL1 antibody CL488-66549Download protocol
Standard Protocols
Click here to view our Standard Protocols