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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CD57 Monoclonal antibody

CD57 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F4A11

Cat no : CL488-66711

Synonyms

B3GAT1, CD57, GlcAT P, GLCATP, GlcUAT P, Glucuronosyltransferase P, HNK 1, HNK1, LEU7, NK 1

Validation Data Gallery

Applications:
  • FC experiment of K-562 using CL488-66711

    FC experiment of K-562 using CL488-66711

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CD57 (CL488-66711, Clone:1F4A11) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inK-562 cells
For other applications, we recommend the unconjugated version of this antibody, 66711-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-66711 targets CD57 in FC (Intra) applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD57 fusion protein Ag13593 相同性解析による交差性が予測される生物種
Full Name beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa, 100 kDa
GenBank accession numberBC010466
Gene symbol B3GAT1
Gene ID (NCBI) 27087
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment. The calculated molecular weight of B3GAT1 is 38 kDa and 66711-1-Ig antibody detectes 100 kDa form in SDS-PAGE which might as a result of homodimer or glycosylation.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD57 antibody CL488-66711Download protocol
Standard Protocols
Click here to view our Standard Protocols