CoraLite® Plus 488 Anti-Mouse CD45R (B220) (RA3-6B2)
CD45R (B220) Monoclonal Antibody for IF/ICC, FC
Host / Isotype
Rat / IgG2a, kappa
Reactivity
mouse
Applications
IF/ICC, FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
RA3-6B2
Cat no : CL488-65139
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | mouse splenocytes |
Positive FC detected in | mouse splenocytes |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-65139 targets CD45R (B220) in IF/ICC, FC applications and shows reactivity with mouse samples.
Tested Reactivity | mouse |
Host / Isotype | Rat / IgG2a, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | Abelson murine leukemia virus-induced pre-B tumor cells 相同性解析による交差性が予測される生物種 |
Full Name | protein tyrosine phosphatase, receptor type, C |
GenBank accession number | BC028512 |
Gene symbol | CD45 |
Gene ID (NCBI) | 19264 |
RRID | AB_2883199 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 CD45R (B220) antibody CL488-65139 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |