CoraLite®555-conjugated CD146/MCAM Monoclonal antibody

CD146/MCAM Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, IF-P

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

4D8A9

Cat no : CL555-66153

Synonyms

CD146, 4D8A9, MUC18, Melanoma-associated antigen MUC18, Melanoma-associated antigen A32


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Tested Applications

Positive IF-P detected inhuman liver cancer tissue
Positive IF/ICC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66153 targets CD146/MCAM in IF/ICC, IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD146/MCAM fusion protein Ag11855 相同性解析による交差性が予測される生物種
Full Name melanoma cell adhesion molecule
Calculated molecular weight 646 aa, 72 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC056418
Gene symbol CD146
Gene ID (NCBI) 4162
RRIDAB_2919675
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.

Protocols

Product Specific Protocols
IF protocol for CL555 CD146/MCAM antibody CL555-66153Download protocol
Standard Protocols
Click here to view our Standard Protocols