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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Alpha Actinin Monoclonal antibody

Alpha Actinin Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E2E1

Cat no : CL488-66895

Synonyms

actinin, alpha 1, ACTN1, Alpha Actinin, Alpha actinin 1, F actin cross linking protein, FLJ40884, FLJ54432, Non muscle alpha actinin 1

Validation Data Gallery

Applications:
  • WB analysis using CL488-66895

    WB analysis using CL488-66895

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66895 (Alpha Actinin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, RAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy Alpha Actinin Ready-To-Use IHC Kit. Alpha Actinin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66895-1-Ig

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Sample-dependent, check data in validation data gallery

Product Information

CL488-66895 targets Alpha Actinin in WB applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Alpha Actinin fusion protein Ag28383 相同性解析による交差性が予測される生物種
Full Name actinin, alpha 1
Calculated molecular weight 103 kDa
Observed molecular weight 100-105 kDa
GenBank accession numberBC015766
Gene symbol ACTN1
Gene ID (NCBI) 87
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 Alpha Actinin antibody CL488-66895Download protocol
Standard Protocols
Click here to view our Standard Protocols