• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Alpha Actinin Polyclonal antibody

Alpha Actinin Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11313

Synonyms

actinin, alpha 1, ACTN1, Alpha actinin 1, F actin cross linking protein, FLJ40884, FLJ54432, Non muscle alpha actinin 1

Validation Data Gallery

Applications:
  • IF Staining of rat heart using CL488-11313

    IF Staining of rat heart using CL488-11313

    Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using CoraLite® Plus 488 Alpha Actinin antibody (CL488-11313) at dilution of 1:200.

  • IF Staining of rat heart using CL488-11313

    IF Staining of rat heart using CL488-11313

    Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using CoraLite® Plus 488 Alpha Actinin antibody (CL488-11313) at dilution of 1:200.

  • FC experiment of A431 using CL488-11313

    FC experiment of A431 using CL488-11313

    1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Alpha Actinin (CL488-11313) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inrat heart tissue
Positive FC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy Alpha Actinin Ready-To-Use IHC Kit. Alpha Actinin primary antibody included.

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-11313 targets Alpha Actinin in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Actinin fusion protein Ag1859 相同性解析による交差性が予測される生物種
Full Name actinin, alpha 1
Calculated molecular weight 103 kDa
Observed molecular weight 100-105 kDa
GenBank accession numberBC015766
Gene symbol ACTN1
Gene ID (NCBI) 87
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Alpha Actinin antibody CL488-11313Download protocol
FC protocol for CL Plus 488 Alpha Actinin antibody CL488-11313Download protocol
Standard Protocols
Click here to view our Standard Protocols