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Acetyl-Histone H4 (Lys12) Recombinant antibody

Acetyl-Histone H4 (Lys12) Recombinant Antibody for WB, IHC, IF/ICC, Dot Blot, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

1D18

Cat no : 83095-1-RR

Synonyms

HIST1H4A, 1D18, Histone-H4, histoneH4, Histone H4


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Tested Applications

Positive WB detected inTrichostatin A treated NIH/3T3 cells, HEK-293 cells
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells
Positive Dot Blot detected inpeptide

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83095-1-RR targets Acetyl-Histone H4 (Lys12) in WB, IHC, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H4a
Observed molecular weight12 kDa
GenBank accession numberBC069654
Gene symbol HIST1H4A
Gene ID (NCBI) 8359
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Acetylation of histone H4 is necessary for chromatin decompaction during DNA replication.

Protocols

Product Specific Protocols
WB protocol for Acetyl-Histone H4 (Lys12) antibody 83095-1-RRDownload protocol
IHC protocol for Acetyl-Histone H4 (Lys12) antibody 83095-1-RRDownload protocol
IF protocol for Acetyl-Histone H4 (Lys12) antibody 83095-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols