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- KD/KO Validated
ATX3,ATXN3 Polyclonal antibody
ATX3,ATXN3 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 13505-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Neuro-2a cells, mouse heart tissue, mouse placenta tissue, HEK-293 cells, mouse brain tissue, rat brain tissue |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 5 publications below |
WB | See 20 publications below |
IHC | See 3 publications below |
IF | See 4 publications below |
IP | See 1 publications below |
CoIP | See 2 publications below |
Product Information
13505-1-AP targets ATX3,ATXN3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, pig, caenorhabditis elegans |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ATX3,ATXN3 fusion protein Ag4341 相同性解析による交差性が予測される生物種 |
Full Name | ataxin 3 |
Calculated molecular weight | 370 aa, 43 kDa |
Observed molecular weight | 35-42 kDa |
GenBank accession number | BC033711 |
Gene symbol | ATXN3 |
Gene ID (NCBI) | 4287 |
RRID | AB_2061192 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.
Protocols
Product Specific Protocols | |
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WB protocol for ATX3,ATXN3 antibody 13505-1-AP | Download protocol |
IHC protocol for ATX3,ATXN3 antibody 13505-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.
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PLoS Genet The Role of the Mammalian DNA End-processing Enzyme Polynucleotide Kinase 3'-Phosphatase in Spinocerebellar Ataxia Type 3 Pathogenesis.
| ||
Sci Total Environ USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway. | ||
PLoS Genet Inactivation of PNKP by mutant ATXN3 triggers apoptosis by activating the DNA damage-response pathway in SCA3. | ||
Neurobiol Dis A new humanized ataxin-3 knock-in mouse model combines the genetic features, pathogenesis of neurons and glia and late disease onset of SCA3/MJD. |