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KD/KO Validated

ALDH1A1 Monoclonal antibody

Name: ALDH1A1 Monoclonal antibody
Brand: Proteintech
SKU: 60171-1-Ig

ALDH1A1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

CloneNo.

1A10A2

Review Product

Cat no : 60171-1-Ig

Synonyms

ALDC, ALDH E1, ALDH1, ALDH11, ALDH1A1, ALHDII, PUMB1, RALDH 1, RALDH1, Retinal dehydrogenase 1

Validation Data Gallery

Applications:

AllWBIHCIFFC

WB analysis of K-562 using 60171-1-Ig
WB result of ALDH1 A1 antibody (60171-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH1 A1 transfected K-562 cells.
WB analysis using 60171-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 60171-1-Ig (ALDH1 A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of MCF-7 using 60171-1-Ig
MCF7 cells were subjected to SDS PAGE followed by western blot with 60171-1-Ig (ALDH1 A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of pig liver using 60171-1-Ig
pig liver tissue were subjected to SDS PAGE followed by western blot with 60171-1-Ig (ALDH1 A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 60171-1-Ig
rat liver tissue were subjected to SDS PAGE followed by western blot with 60171-1-Ig (ALDH1 A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human brain using 60171-1-Ig
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60171-1-Ig (ALDH1 A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).
IHC staining of human brain using 60171-1-Ig
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60171-1-Ig (ALDH1 A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).
IF Staining of HeLa using 60171-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ALDH1 A1 antibody (60171-1-Ig, Clone: 1A10A2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of mouse colon using 60171-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using 60171-1-Ig (ALDH1 A1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of mouse colon using 60171-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using 60171-1-Ig (ALDH1 A1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of A431 using 60171-1-Ig
Immunofluorescent analysis of A431 cells using 60171-1-Ig (ALDH1 A1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.
FC experiment of HepG2 using 60171-1-Ig
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human ALDH1 A1 (60171-1-Ig, Clone:1A10A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 60171-1-Ig

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, MCF-7 cells, pig liver tissue, rat liver tissue, K-562 cells, HepG2 cells, HuH-7 cells
Positive IHC detected in	human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HeLa cells, A431 cells, mouse colon tissue
Positive FC detected in	HepG2 cells

Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:400-1:1600
Flow Cytometry (FC)	FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 8 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below
FC	See 1 publications below
CoIP	See 1 publications below

Product Information

60171-1-Ig targets ALDH1A1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Cited Reactivity	human, mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	ALDH1A1 fusion protein Ag8551 相同性解析による交差性が予測される生物種
Full Name	aldehyde dehydrogenase 1 family, member A1
Calculated molecular weight	501 aa, 55 kDa
Observed molecular weight	52 kDa
GenBank accession number	BC001505
Gene symbol	ALDH1A1
Gene ID (NCBI)	216
RRID	AB_10693634
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1A1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001).

Protocols

Product Specific Protocols
WB protocol for ALDH1A1 antibody 60171-1-Ig	Download protocol
IHC protocol for ALDH1A1 antibody 60171-1-Ig	Download protocol
IF protocol for ALDH1A1 antibody 60171-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Cell Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice. Authors - Haibo Zhou View Article
human	WB,CoIP	iScience Modification of lysine-260 2-hydroxyisobutyrylation destabilizes ALDH1A1 expression to regulate bladder cancer progression Authors - Zhilei Zhang KD Validated View Article
human	WB	Front Pharmacol Autophagy Inhibition Enhances the Anti-Tumor Activity of Methylseleninic Acid in Cisplatin-Resistance Human Lung Adenocarcinoma Cells. Authors - Ming Xin View Article
human	WB	Oral Dis microRNA-17 is a tumor suppressor in oral squamous cell carcinoma and is repressed by LSD1. Authors - Kaixin Wangzhou View Article
human	WB	Mol Med Rep lncRNA TPTEP1 inhibits stemness and radioresistance of glioma through miR‑106a‑5p‑mediated P38 MAPK signaling. Authors - Ting Tang View Article
human	WB	Cancer Cell Int PMID: 31139022 Authors - Rui-Qi Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALDH1A1 Monoclonal antibody

ALDH1A1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Validation Data Gallery

WB analysis of K-562 using 60171-1-Ig

WB analysis using 60171-1-Ig

WB analysis of MCF-7 using 60171-1-Ig

WB analysis of pig liver using 60171-1-Ig

WB analysis of rat liver using 60171-1-Ig

IHC staining of human brain using 60171-1-Ig

IHC staining of human brain using 60171-1-Ig

IF Staining of HeLa using 60171-1-Ig

IF Staining of mouse colon using 60171-1-Ig

IF Staining of mouse colon using 60171-1-Ig

IF Staining of A431 using 60171-1-Ig

FC experiment of HepG2 using 60171-1-Ig

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.

Modification of lysine-260 2-hydroxyisobutyrylation destabilizes ALDH1A1 expression to regulate bladder cancer progression

Autophagy Inhibition Enhances the Anti-Tumor Activity of Methylseleninic Acid in Cisplatin-Resistance Human Lung Adenocarcinoma Cells.

microRNA-17 is a tumor suppressor in oral squamous cell carcinoma and is repressed by LSD1.

lncRNA TPTEP1 inhibits stemness and radioresistance of glioma through miR‑106a‑5p‑mediated P38 MAPK signaling.

PMID: 31139022

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