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CoraLite® Plus 488-conjugated Alpha Tubulin Polyclonal antibody

Alpha Tubulin Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14555

Synonyms

alpha tubulin, Alpha tubulin 3, B ALPHA 1, FLJ25113, LIS3, TUBA1A, TUBA3, Tubulin alpha 1A chain, Tubulin alpha 3 chain, Tubulin B alpha 1, tubulin, alpha 1a

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated Alpha Tubulin Polycl (CL488-14555)

    IF Staining of HeLa using CL488-14555

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Alpha Tubulin antibody (CL488-14555) at dilution of 1:200.

Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14555 targets Alpha Tubulin in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Tubulin fusion protein Ag6038
Full Name tubulin, alpha 1a
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC050637
Gene Symbol TUBA1A
Gene ID (NCBI) 7846
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Alpha Tubulin antibody CL488-14555Download protocol
Standard Protocols
Click here to view our Standard Protocols