C4-B Alpha Chain/C4b-B/C4d-B Polyclonal antibody

C4-B Alpha Chain/C4b-B/C4d-B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 55228-1-AP

Synonyms

C4, C4B, C4B1, C4B12, C4B2, C4B3, C4B5, C4F, CH, CO4, Complement C4-B, CPAMD3



Tested Applications

Positive WB detected inhuman blood

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55228-1-AP targets C4-B Alpha Chain/C4b-B/C4d-B in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name complement component 4B (Chido blood group)
Calculated Molecular Weight 193 kDa
Observed Molecular Weight 95 kDa, 300 kDa
GenBank Accession NumberNM_000592
Gene Symbol C4B
Gene ID (NCBI) 721
RRIDAB_2881291
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular wights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1116-1128aa of human C4-B protein can recognize C4-B precursor, C4-B alpha chain, C4b-B, and C4d-B.

Protocols

Product Specific Protocols
WB protocol for C4-B Alpha Chain/C4b-B/C4d-B antibody 55228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols