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KD/KO Validated

USP5 Polyclonal antibody

Name: USP5 Polyclonal antibody
Brand: Proteintech
SKU: 10473-1-AP

USP5 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10473-1-AP

Synonyms

Deubiquitinating enzyme 5, Isopeptidase T, ISOT, Ubiquitin thiolesterase 5, USP5

Validation Data Gallery

Applications:

AllWBIPIHC

WB analysis of A549 using 10473-1-AP
WB result of USP5 antibody (10473-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP5 transfected A549 cells.
WB analysis using 10473-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 10473-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 10473-1-AP
human colon tissue were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of DU 145 using 10473-1-AP
DU 145 cells were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10473-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 10473-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MDA-MB-453s using 10473-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of U2OS using 10473-1-AP
U2OS cells were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 10473-1-AP
IP result of anti-USP5 (IP:10473-1-AP, 4ug; Detection:10473-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human heart using 10473-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10473-1-AP (USP5 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human heart using 10473-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10473-1-AP (USP5 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human spleen using 10473-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10473-1-AP (USP5 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human spleen using 10473-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10473-1-AP (USP5 antibody) at dilution of 1:200 (under 40x lens).

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Cat No. 10473-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, mouse brain tissue, human heart tissue, human colon tissue, U2OS cells, DU 145 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human heart tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 12 publications below
WB	See 30 publications below
IHC	See 5 publications below
IF	See 4 publications below
IP	See 4 publications below
ELISA	See 2 publications below
CoIP	See 1 publications below

Product Information

10473-1-AP targets USP5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	USP5 fusion protein Ag0770 相同性解析による交差性が予測される生物種
Full Name	ubiquitin specific peptidase 5 (isopeptidase T)
Calculated molecular weight	96 kDa
Observed molecular weight	95-105 kDa
GenBank accession number	BC004889
Gene symbol	USP5
Gene ID (NCBI)	8078
RRID	AB_2272754
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. USP5 has 2 isoforms with the molecular mass of 96 kDa and 93 kDa.

Protocols

Product Specific Protocols
WB protocol for USP5 antibody 10473-1-AP	Download protocol
IHC protocol for USP5 antibody 10473-1-AP	Download protocol
IP protocol for USP5 antibody 10473-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Commun STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity Authors - Hiroaki Ohishi View Article
		Blood The ubiquitin ligase HERC4 mediates c-Maf ubiquitination and delays the growth of multiple myeloma xenografts in nude mice. Authors - Zubin Zhang View Article
human	WB	Theranostics Ubiquitin specific peptidase 5 regulates colorectal cancer cell growth by stabilizing Tu translation elongation factor. Authors - Xin Xu KD Validated View Article
human	WB	Clin Transl Med MAFG-AS1 promotes tumor progression via regulation of the HuR/PTBP1 axis in bladder urothelial carcinoma. Authors - Mengqing Xiao KD Validated View Article
human	WB	Acta Pharmacol Sin Mebendazole elicits potent antimyeloma activity by inhibiting the USP5/c-Maf axis. Authors - Xue-Han Chen View Article
rat	WB,IP	Sci Signal Selective inhibition of CaV3.2 channels reverses hyperexcitability of peripheral nociceptors and alleviates postsurgical pain. Authors - Sonja L Joksimovic View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP5 Polyclonal antibody

USP5 Polyclonal Antibody for IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis of A549 using 10473-1-AP

WB analysis using 10473-1-AP

WB analysis of A549 using 10473-1-AP

WB analysis of human colon using 10473-1-AP

WB analysis of DU 145 using 10473-1-AP

WB analysis of mouse brain using 10473-1-AP

WB analysis of human heart using 10473-1-AP

WB analysis of MDA-MB-453s using 10473-1-AP

WB analysis of U2OS using 10473-1-AP

IP experiment of mouse brain using 10473-1-AP

IHC staining of human heart using 10473-1-AP

IHC staining of human heart using 10473-1-AP

IHC staining of human spleen using 10473-1-AP

IHC staining of human spleen using 10473-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity

The ubiquitin ligase HERC4 mediates c-Maf ubiquitination and delays the growth of multiple myeloma xenografts in nude mice.

Ubiquitin specific peptidase 5 regulates colorectal cancer cell growth by stabilizing Tu translation elongation factor.

MAFG-AS1 promotes tumor progression via regulation of the HuR/PTBP1 axis in bladder urothelial carcinoma.

Mebendazole elicits potent antimyeloma activity by inhibiting the USP5/c-Maf axis.

Selective inhibition of CaV3.2 channels reverses hyperexcitability of peripheral nociceptors and alleviates postsurgical pain.