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  • Featured Product
  • KD/KO Validated

TFEB Polyclonal antibody

TFEB Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IF, IHC, CoIP, ELISA, Wes Immunoassay

Conjugate

Unconjugated

Cat no : 13372-1-AP

Synonyms

AlphaTFEB, bHLHe35, TCFEB, TFEB, transcription factor EB

Validation Data Gallery

Applications:
  • WB analysis using 13372-1-AP

    WB analysis using 13372-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 13372-1-AP

    WB analysis of Jurkat using 13372-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 13372-1-AP

    WB analysis using 13372-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 13372-1-AP

    WB analysis of Jurkat using 13372-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 13372-1-AP

    WB analysis of HeLa using 13372-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human placenta using 13372-1-AP

    WB analysis of human placenta using 13372-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 13372-1-AP

    WB analysis of NIH/3T3 using 13372-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat brain using 13372-1-AP

    WB analysis of rat brain using 13372-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Raji using 13372-1-AP

    IP experiment of Raji using 13372-1-AP

    IP result of anti-TFEB (IP:13372-1-AP, 4ug; Detection:13372-1-AP 1:10000) with Raji cells lysate 2800 ug.

  • IHC staining of human renal cell carcinoma using 13372-1-AP

    IHC staining of human renal cell carcinoma using 13372-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13372-1-AP (TFEB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TFEB antibody included
  • IHC staining of human renal cell carcinoma using 13372-1-AP

    IHC staining of human renal cell carcinoma using 13372-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13372-1-AP (TFEB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TFEB antibody included
  • IF Staining of HeLa using 13372-1-AP

    IF Staining of HeLa using 13372-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TFEB antibody (13372-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, Jurkat cells, HeLa cells, NIH/3T3 cells, human placenta tissue, rat brain tissue, HEK-293n cells, Raji cells, C2C12 cells, RAW 264.7 cells, mouse heart tissue, rat heart tissue
Positive IP detected inRaji cells
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TFEB Ready-To-Use IHC Kit. TFEB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13372-1-AP targets TFEB in WB, IP, IF, IHC, CoIP, ELISA, Wes Immunoassay applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse, monkey, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TFEB fusion protein Ag4194 相同性解析による交差性が予測される生物種
Full Name transcription factor EB
Calculated molecular weight 476 aa, 53 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC032448
Gene symbol TFEB
Gene ID (NCBI) 7942
RRIDAB_2199611
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calcualted molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).

Protocols

Product Specific Protocols
WB protocol for TFEB antibody 13372-1-APDownload protocol
IHC protocol for TFEB antibody 13372-1-APDownload protocol
IF protocol for TFEB antibody 13372-1-APDownload protocol
IP protocol for TFEB antibody 13372-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

ACS Nano

A Self-Assembled α-Synuclein Nanoscavenger for Parkinson's Disease.

Authors - Jingyi Liu
View Article
mouseIF

Acta Neuropathol

Wolframin is a novel regulator of tau pathology and neurodegeneration.

Authors - Shuo Chen
View Article
mouseWB,IF,IP

Mol Cell

Itaconate is a lysosomal inducer that promotes antibacterial innate immunity.

Authors - Zhenxing Zhang
View Article
mouseWB,IF

J Hazard Mater

VPS41-mediated incomplete autophagy aggravates cadmium-induced apoptosis in mouse hepatocytes

Authors - Tao Wang
View Article
humanWB

Cell Death Differ

Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration

Authors - Huaizhen Liang
  • KD Validated
View Article
mouseWB

Mol Psychiatry

TFEB regulates lysosomal exocytosis of tau and its loss of function exacerbates tau pathology and spreading.

Authors - Yin Xu
  • KO Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Shunbin (Verified Customer) (07-17-2023)

bands look specific, but blurry and multiple.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: B cell lines
TFEB Antibody Western Blot validation (1:2000 dilution) in B cell lines (Cat no:13372-1-AP)
FH

Weihao (Verified Customer) (04-11-2022)

Good antibody for mouse BMDM! not for human MDM 1:500 for IF 1:2000 for WB

  • Applications: Western Blot, Immunofluorescence
  • Cell Tissue Type: mouse BMDM
FH

Marina (Verified Customer) (11-22-2021)

2 bands visible, unclear which is the right one, although size is ok-ish for both. Both bands are quite weak.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500 in 1% skim milk in TBST
  • Cell Tissue Type: primary mouse microglia
FH

Tom (Verified Customer) (02-19-2021)

HEK293 lysate (10ug) subjected to SDS-PAGE and transferred to nitrocellullose. Membrane blocked in 5% BSA for 1 hour prior to incubation with antibody (1:1000 in block) overnight at 4 degrees. Band detected using anti-rabbit HRP secondary.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293
TFEB Antibody Western Blot validation (1:1000 dilution) in HEK293 (Cat no:13372-1-AP)