- Featured Product
- KD/KO Validated
SIK1 Polyclonal antibody
SIK1 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, Rat and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 51045-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells |
| Positive IHC detected in | mouse brain tissue, human liver tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 13 publications below |
| IHC | See 3 publications below |
| IF | See 6 publications below |
Product Information
51045-1-AP targets SIK1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, Rat samples.
| Tested Reactivity | human, mouse, Rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SIK1 fusion protein Ag3913 相同性解析による交差性が予測される生物種 |
| Full Name | salt-inducible kinase 1 |
| Calculated molecular weight | 783 aa, 85 kDa |
| Observed molecular weight | 85-100 kDa |
| GenBank accession number | BC038504 |
| Gene symbol | SIK1 |
| Gene ID (NCBI) | 150094 |
| RRID | AB_2187322 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SIK1(Salt-inducible kinase 1) is also named as SIK, SNF1LK and belongs to the CAMK Ser/Thr protein kinase family. It associates constitutively with the NK regulatory complex and is responsible for increases in its catalytic activity following small elevations in intracellular sodium concentrations(PMID:17939993). SIK1 is required for phosphorylation of class II HDACs and for expression of MEF2 target genes in myocytes(PMID:17468767). Defects in SIK1 may be associated with some cancers, such as breast cancers. Loss of SIK1 correlates with poor patient outcome in breast cancers(PMID:19622832). SIK1 also can be detected 95-100 kDa (PMID: 26567857, PMID: 22109884).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SIK1 antibody 51045-1-AP | Download protocol |
| IHC protocol for SIK1 antibody 51045-1-AP | Download protocol |
| IF protocol for SIK1 antibody 51045-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Phytomedicine Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis | ||
J Neuroinflammation High-salt diet downregulates TREM2 expression and blunts efferocytosis of macrophages after acute ischemic stroke. | ||
J Cell Physiol ETNPPL modulates hyperinsulinemia-induced insulin resistance through the SIK1/ROS-mediated inactivation of the PI3K/AKT signaling pathway in hepatocytes | ||
J Cereb Blood Flow Metab Role of salt-induced kinase 1 in androgen neuroprotection against cerebral ischemia. | ||
J Leukoc Biol SIK inhibition in human myeloid cells modulates TLR and IL-1R signaling and induces an anti-inflammatory phenotype. | ||
Inflamm Bowel Dis HG-9-91-01 Attenuates Murine Experimental Colitis by Promoting Interleukin-10 Production in Colonic Macrophages Through the SIK/CRTC3 Pathway. |
