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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

SAMSN1 Polyclonal antibody

SAMSN1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13063-1-AP

Synonyms

HACS1, NASH1, SAMSN1, SASH2, SH3 SAM adaptor protein, SH3D6B

Validation Data Gallery

Applications:
  • WB analysis of K-562 using 13063-1-AP

    WB analysis of K-562 using 13063-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13063-1-AP (SAMSN1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 13063-1-AP

    WB analysis of K-562 using 13063-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13063-1-AP (SAMSN1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 13063-1-AP

    IP experiment of K-562 using 13063-1-AP

    IP result of anti-SAMSN1 (IP:13063-1-AP, 4ug; Detection:13063-1-AP 1:1000) with K-562 cells lysate 4000 ug.

  • IHC staining of human gliomas using 13063-1-AP

    IHC staining of human gliomas using 13063-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13063-1-AP (SAMSN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SAMSN1 antibody included
  • IHC staining of human gliomas using 13063-1-AP

    IHC staining of human gliomas using 13063-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13063-1-AP (SAMSN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SAMSN1 antibody included
  • IF Staining of HepG2 using 13063-1-AP

    IF Staining of HepG2 using 13063-1-AP

    Immunofluorescent analysis of HepG2 cells using 13063-1-AP (SAMSN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SAMSN1 Ready-To-Use IHC Kit. SAMSN1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13063-1-AP targets SAMSN1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAMSN1 fusion protein Ag3755 相同性解析による交差性が予測される生物種
Full Name SAM domain, SH3 domain and nuclear localization signals 1
Calculated molecular weight 42 kDa
Observed molecular weight 42-50 kDa
GenBank accession numberBC029112
Gene symbol SAMSN1
Gene ID (NCBI) 64092
RRIDAB_2877910
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SAMSN1 antibody 13063-1-APDownload protocol
IHC protocol for SAMSN1 antibody 13063-1-APDownload protocol
IF protocol for SAMSN1 antibody 13063-1-APDownload protocol
IP protocol for SAMSN1 antibody 13063-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Stem Cells Int

Lama2 And Samsn1 Mediate the Effects of Brn4 on Hippocampal Neural Stem Cell Proliferation and Differentiation

Authors - Lei Zhang
  • KD Validated
View Article
humanIHC

Ann Surg Oncol

Suppression of SAMSN1 Expression is Associated with the Malignant Phenotype of Hepatocellular Carcinoma.

Authors - Satoshi Sueoka
View Article
humanIHC

Oncol Lett

Prognostic relevance of SAMSN1 expression in gastric cancer.

Authors - Mitsuro Kanda
View Article