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RPS3 Monoclonal antibody
RPS3 Monoclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ChIP, ELISA
Conjugate
Unconjugated
CloneNo.
2G7H4
Cat no : 66046-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HSC-T6 cells, HeLa cells, human brain tissue, Neuro-2a cells, RAW 264.7 cells, ROS1728 cells, rat brain tissue, HEK-293 cells, HepG2 cells, K-562 cells, Jurkat cells, NIH/3T3 cells, 4T1 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human ovary tumor tissue, human colon cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
| FC | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
66046-1-Ig targets RPS3 in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RPS3 fusion protein Ag7515 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein S3 |
| Calculated molecular weight | 27 kDa |
| Observed molecular weight | 33 kDa |
| GenBank accession number | BC003137 |
| Gene symbol | RPS3 |
| Gene ID (NCBI) | 6188 |
| RRID | AB_11182493 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival. The calculated molecular weight of RPS3 is 26 kDa, but the modified RPS3 is about 33 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPS3 antibody 66046-1-Ig | Download protocol |
| IHC protocol for RPS3 antibody 66046-1-Ig | Download protocol |
| IF protocol for RPS3 antibody 66046-1-Ig | Download protocol |
| IP protocol for RPS3 antibody 66046-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Transl Res Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication. | ||
PLoS Pathog Separate domains of G3BP promote efficient clustering of alphavirus replication complexes and recruitment of the translation initiation machinery. | ||
Free Radic Biol Med Identification of interacting partners of Human Mpv17-like protein with a mitigating effect of mitochondrial dysfunction through mtDNA damage. | ||
Oncotarget Ribosomal protein S3 (rpS3) secreted from various cancer cells is N-linked glycosylated.
| ||
