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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

RHBDD1 Polyclonal antibody

RHBDD1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20869-1-AP

Synonyms

HSD 50, RHBDD1, RHBDL4, rhomboid domain containing 1

Validation Data Gallery

Applications:
  • WB analysis of mouse kidney using 20869-1-AP

    WB analysis of mouse kidney using 20869-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20869-1-AP (RHBDD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 20869-1-AP

    WB analysis using 20869-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20869-1-AP (RHBDD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 20869-1-AP

    WB analysis of HEK-293 using 20869-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20869-1-AP (RHBDD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 20869-1-AP

    WB analysis of PC-3 using 20869-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20869-1-AP (RHBDD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 20869-1-AP

    WB analysis of mouse testis using 20869-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20869-1-AP (RHBDD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 20869-1-AP

    IHC staining of human testis using 20869-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20869-1-AP (RHBDD1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 20869-1-AP

    IHC staining of human testis using 20869-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20869-1-AP (RHBDD1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells, mouse testis tissue, PC-3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20869-1-AP targets RHBDD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RHBDD1 fusion protein Ag14932 相同性解析による交差性が予測される生物種
Full Name rhomboid domain containing 1
Calculated molecular weight 315 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC027900
Gene symbol RHBDD1
Gene ID (NCBI) 84236
RRIDAB_10733756
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RHBDD1 antibody 20869-1-APDownload protocol
IHC protocol for RHBDD1 antibody 20869-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Biol Chem

AAA-ATPase valosin-containing protein (VCP) binds the transcription factor SREBP1 and promotes its proteolytic activation by rhomboid protease RHBDL4.

Authors - Koji Shibuya
View Article
humanWB

Environ Toxicol

Suppression of rhomboid domain-containing 1 produces anticancer effects in pancreatic adenocarcinoma through affection of the AKT/GSK-3β/β-catenin pathway.

Authors - Jiong Jiang
  • KD Validated
View Article
humanWB

Cell Death Dis

Long noncoding RNA AFAP1-AS1 promotes tumor progression and invasion by regulating the miR-2110/Sp1 axis in triple-negative breast cancer.

Authors - Xiaohui Zhang
View Article
humanWB

Genes Cells

Mouse transient receptor potential melastatin 2 (TRPM2) isoform 7 attenuates full-length mouse TRPM2 activity through reductions in its expression by targeting it to ER-associated degradation

Authors - Shinichiro Yamamoto
  • KD Validated
View Article