Mouse VEGF ELISA Kit0 Publications

カタログ番号: KE10009

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Mouse VEGF ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
2.0 pg/mL

測定範囲:
7.8-500 pg/mL

測定可能動物種:
Mouse

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 94% 92%-98%
Mouse serum 93% 74%-110%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 37.0 2.5 3.4
2 20 118.0 9.0 3.8
3 20 369.0 63.6 8.6

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 39.0 4.5 5.7
2 24 128.0 7.1 2.8
3 24 390.0 31.9 4.1

製品概要
KE10009 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse VEGF ELISA kit is to be used to detect and quantify protein levels of endogenous mouse VEGF. The assay recognizes mouse VEGF. A polyclonal antibody specific for mouse VEGF has been pre-coated onto the microwells. The mouse VEGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for mouse VEGF is added to detect the captured mouse VEGF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Vegf, Vegf a, Vegf120, Vegf164, Vegf188, Vegfa, Vpf
背景説明

Vascular endothelial growth factor (VEGF), is a signal protein produced by cells that stimulates vasculogenesis and angiogenesis. It is part of the system that restores the oxygen supply to tissues when blood circulation is inadequate such as in hypoxic conditions. Serum concentration of VEGF is high in bronchial asthma and diabetes mellitus. The activities of VEGF are not limited to the vascular system; VEGF plays a role in normal physiological functions such as bone formation, hematopoiesis, wound healing, and development. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. VEGF is upregulated in many known tumors and its expression is correlated with tumor stage and progression.


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