Mouse IL21 ELISA Kit0 Publications

カタログ番号: KE10012

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Mouse IL21 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
0.5 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Mouse

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 76% 71%-86%
Mouse serum 92% 77%-104%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 74.7 4.8 6.5
2 20 183.6 12.4 6.7
3 20 717.5 38.4 5.4

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 77.7 6.9 8.9
2 24 198.3 9.4 4.7
3 24 941.5 89.4 9.5

製品概要
KE10012 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL21 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL21. The assay recognizes mouse IL21. A monoclonal antibody specific for mouse IL21 has been pre-coated onto the microwells. The mouse IL21 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for mouse IL21 is added to detect the captured mouse IL21 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Il21, IL-21, interleukin 21, Interleukin-21
背景説明

Interleukin 21 (IL21) is a cytokine produced predominantly by cluster of differentiation 4 (CD4+) T-cells and natural killer T-cells. IL21 is preliminarily expressed in activated cluster of differentiation 4 (CD4+) T-cells and natural killer (NK) T-cells. IL21 receptor (il21R) is a class I cytokine receptor, which requires dimerization with the indispensable common gamma chain (γc) subunit to bind IL21. IL21 plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. IL21 mediates apoptosis in B-CLL cells through up-regulation of the BIM (BH3 family member) and enhances both direct and antibody-dependent cellular cytotoxicity in these cells.


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