Human TF ELISA Kit0 Publications

カタログ番号: KE00046

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

販売および在庫状況はコスモ・バイオ(株)にお問い合わせください。
distributor

Images See all

概要


製品名:
Human TF ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant, Urine

アッセイ方法:
Sandwich

感度:
0.6 ng/mL

測定範囲:
1.56-100 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 102% 90%-120%
Urine 88% 79%-97%
Human plasma 103% 81%-126%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 111.4 5.6 5.0
2 20 18.4 0.8 4.2
3 20 4.5 0.2 5.3

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 101.3 8.2 8.1
2 24 17.9 1.3 7.2
3 24 4.3 0.3 7.5

製品概要
KE00046 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TF ELISA kit is to be used to detect and quantify protein levels of endogenous TF. The assay recognizes human TF. A polyclonal antibody specific for TF has been pre-coated onto the microwells. The TF protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for TF is added to detect the captured TF protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Beta 1 metal binding globulin, PRO1557, PRO2086, Serotransferrin, Siderophilin, TF, transferrin
背景説明

Serotransferrin (TF) is a 77 kDa secreted protein which is also named as transferrin, siderophilin, beta-1 metal-binding globulin and belongs to a family of homologous iron-binding glycoproteins that encompasses lactoferrin (found both intracellular and in secretions, including milk), melanotrasferrin (present on melanoma cells) and ovotransferrin (present in egg white). TF is a multi-function protein with a primary role in transporting iron in a safe, redox-inactive state from absorption to utilization or storage sites around the body. The association of Tf with the immune system derives from its ability to restrict serum free-iron levels, creating low-iron environments where the infection capacity of pathogenic microorganisms is limited. TF is expressed predominantly in the liver and secreted in plasma, but lower amounts can be synthesized in other tissues such as the brain and the testis.


Back
to top