Human P21 ELISA Kit0 Publications

カタログ番号: KE00049

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human P21 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Cell lysate

アッセイ方法:
Sandwich

感度:
0.1 ng/mL

測定範囲:
0.25-16 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell lysate 87% 81%-97%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 10.29 0.201 2.0
2 20 3.05 0.070 2.3
3 20 0.63 0.025 4.0

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 10.24 0.346 3.4
2 24 3.08 0.094 3.1
3 24 0.67 0.052 7.8

製品概要
KE00049 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The P21 ELISA kit is designed for the quantitative determination of P21 in samples of human and mouse origin. A polyclonal antibody specific for P21 has been pre-coated onto the microwells. The P21 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for P21 is added to detect the captured P21 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, P21;CDKN1A, p21CIP1, SDI1, WAF1
背景説明

CDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. p21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. p21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. p21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes.


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