Human ORM1 ELISA Kit0 Publications

カタログ番号: KE00066

93,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human ORM1 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Cell culture supernatant, Urine, Human plasma

アッセイ方法:
Sandwich

感度:
0.2 pg/mL

測定範囲:
31.25-2000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 96% 89%-111%
Urine 79% 71%-94%
Human plasma 96% 82%-114%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 147.4 8.9 6.0
2 20 431.6 15.3 3.5
3 20 1,500.4 51.8 3.5

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 145.5 11.5 7.9
2 24 453.6 31.0 6.8
3 24 1,536.1 78.7 5.1

製品概要
KE00066 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ORM1 ELISA kit is to be used to detect and quantify protein levels of endogenous ORM1. The assay recognizes human ORM1. A polyclonal antibody specific for ORM1 has been pre-coated onto the microwells. The ORM1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for ORM1 is added to detect the captured ORM1 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
AGP 1, AGP A, AGP1, Alpha 1 acid glycoprotein 1, OMD 1, ORM, ORM1, orosomucoid 1
背景説明

ORM1 (Orosomucoid 1), also named AGP1 (Alpha-1-acid glycoprotein 1) and AGP A, is an abundant plasma protein characterized by anti-inflammatory and immune-modulating properties. In human, two isoforms have been described, named ORM1 and ORM2. ORM1 is implicated in two main different functions: (1) as a lipocalin family member, it exerts a carrier activity especially for drug and lipid molecules; (2) being an acute phase protein (PMID: 23973664). ORM1 are increased in the patients with cancers, burnt injury or surgical operation (PMID: 10913518; PMID: 1646598). ORM1 could be a potential novel urinary biomarker for the early detection of chronic heart failure (PMID: 25215505).


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