Human MHC class I ELISA Kit2 Publications

カタログ番号: KE00035

93,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human MHC class I ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma

アッセイ方法:
Sandwich

感度:
19.6 pg/mL

測定範囲:
31.25-2000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human plasma 103% 85%-122%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 126.8 10.1 8.0
2 20 942.0 67.2 7.1
3 20 1,416.3 101.3 7.2

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 146.6 15.2 10.4
2 24 1,085.7 72.1 6.6
3 24 1901.1 100.8 5.3

製品概要
KE00035 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MHC class Ⅰ ELISA Kit is to be used to detect and quantify protein levels of endogenous MHC class Ⅰ molecules. The assay recognizes human MHC class Ⅰ molecules. A polyclonal MHC class I antibody has been pre-coated onto the microwells. The MHC class Ⅰ proteins in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal MHC class I antibody is added to detect the captured MHC class Ⅰ proteins. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
HLA A, HLA class I (HLA-A), HLAA, HLA-A, HLA-B, HLA-C, MHC class I antigen A*3
背景説明

Major histocompatibility complex (MHC) antigens, also know in human as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). MHC class I molecules (HLA-A, -B and -C) are polymorphic membrane glycoproteins composed of two polypeptide chains, a heavy alpha chain encoded by variant HLA-A, -B or -C gene and a smaller beta chain (β2-microglobulin). They are peptide-binding proteins that present processed antigens to T lymphocytes.


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